Coding

Part:BBa_K2663001:Design

Designed by: Havisha Pedamallu   Group: iGEM18_WashU_StLouis   (2018-10-17)


Ribitol Transporter Operon


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 67
    Illegal SpeI site found at 1363
    Illegal PstI site found at 1375
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 67
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 1363
    Illegal PstI site found at 1375
    Illegal NotI site found at 73
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 67
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 67
    Illegal SpeI site found at 1363
    Illegal PstI site found at 1375
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 67
    Illegal XbaI site found at 82
    Illegal SpeI site found at 1363
    Illegal PstI site found at 1375
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

The sequence for the ribitol transporter comes from E. coli 1303 and the promoter and RBS came from the registry.

References